Physico-Chemical Methods Applied To Bioactive Compounds Determination From Ceramium Rubrum On The Romanian Coast Of The Black Sea

Abstract

The red seaweed species Ceramium rubrum found in abundance along the Romanian Black Sea coast. Phylum Rhodophyta, Class Florideophycidae, Family Ceramiaceae, Genus Ceramium to which the species Ceramium rubrum belongs, is recognized due to two compounds: agar-agar and carrageenan which are widely used in the medical, pharmaceutical and food industry for their properties, agar-agar and carrageenan. The study aims to determine and quantify the active principles in the red seaweed Ceramium rubrum using IR and UV-VIS spectrometry and chromatographic methods. For IR spectrometry, dry algal powder and standard β-carotene used. For simultaneous evaluation of β-carotene, chlorophyll a and chlorophyll b, a chromatographic method (high performance liquid chromatography) applied. The antioxidant capacity analyzed by chemiluminescence method, showing remarkable results.